Coding

Part:BBa_K1218019:Design

Designed by: Alex Constantino   Group: iGEM13_Stanford-Brown   (2013-08-30)


CASCADE Complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BglII site found at 4279
    Illegal BamHI site found at 23
    Illegal BamHI site found at 3224
    Illegal BamHI site found at 3585
    Illegal BamHI site found at 4482
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5203
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1764


Design Notes

Silent mutations were engineered via site-directed mutagenesis to remove two internal PstI sites.


Source

Amplified from DH5α genomic DNA.

References